Boosting Recombinant Inclusion Body Production—From Classical Fed-Batch Approach to Continuous Cultivation

Production of recombinant human annexin V by fed-batch cultivation

BACKGROUND Annexin V, a 35.8 kDa intracellular protein, is a Ca⁺²-dependent phospholipid binding protein with high affinity to phosphatidylserine (PS), which is a well-known hallmark of apoptosis. Annexin V is a sensitive probe for PS exposure upon the cell membrane, and used for detection of apoptotic cells both in vivo and in vitro. Large-scale production of recombinant human annexin V is wor...

Fed-batch Cultivation of Recombinant Escherichia coli Producing Human Interferon-γ Under Controlled Specific Growth Rate

Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch

Background Visceral leishmaniasis is an infecto-parasitarian disease caused by obligatory intracellular protozoa belonging to the genus Leishmania, and might be lethal since there is no precocious diagnosis and proper treatment. Despite the considerable effort, there is no effective and safe vaccine for human use [1]. Genetically modified microorganisms are used industrially in general for prod...

Optimisation Methods for Improving Fed-batch Cultivation of E. Coli Producing Recombinant Proteins

Two optimisation techniques for the fed-batch cultivation of high cell density Escherichia coli producing recombinant proteins were compared. An unstructured model for the growth, based on the General State Space Dynamical Model [1] was used to represent the four major metabolic pathways: oxidative growth on glucose, fermentative growth on glucose, oxidative growth on acetate, and maintenance. ...

Multiple model approach to modelling of Escherichia coli fed-batch cultivation extracellular production of bacterial phytase